Processive enzymes are proteins that catalyze consecutive reactions without releasing its substrate.
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An example of processive enzymes is RNA polymerase which binds to a DNA strand and repeatedly catalyzes nucleotide transfers, effectively synthesizing a corresponding RNA strand.
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Nolte and coworkers developed an artificial processive enzyme in a form of manganese porphyrin rotaxane that treads along a long polymer of alkene and catalyze multiple rounds of alkene epoxidation.
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One challenge for the'exonuclease approach', where a processive enzyme feeds individual bases, in the correct order, into the nanopore, is to integrate the exonuclease and the nanopore detection systems.
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